| 1. | Based on these results , we suggest a method for c - terminal sequencing of a protein
基于这些结果，我们提出了蛋白质c端测序的方法。 |
| 2. | The chemical degradation method established by edman in 1950 has been a routine approach for n - terminal sequencing and is widely employed by automated protein sequencer
运用edman降解进行蛋白质n端顺序测定已成为十分完善的技术，并已经实现了自动化。 |
| 3. | Its molecular weight was determined to be 26 . 8kda by sds - page and the same under reduced conditions . its anti - coagulation activity was characterized . the n - terminal sequence of tsk - 2 is rtefqrymeiw
该组分为单链蛋白质，分子量为26 . 8kda ，具有抗凝血活性，是嗜硫色谱洗脱峰降解后新产生的蛋白组分。 |
| 4. | It is shown that the obtained proteins ( hq - 5 & tsk - 2 ) can be identified as novel components of anti - coagulation enzyme family in the venom of agkistrodon acutus by the analysis of the n - terminal sequence of tsk - 2
其一级序列为rtefqrymeivv 。与从蕲蛇蛇毒中分离出的其它组分相比， hq - 5及其降解产生的组分( tsk - 2 )为新的抗凝血组分。 |
| 5. | The sequences flanking tn5 in magnetosome deleted mutant nm4 was cloned by anchored pcr , and was used as tag to extend the 5 ' and 3 ' terminal sequences of the target dna fragment by anchored pcr constinuously
以tn5为标签，用锚定pcr法克隆出突变株nm4的tn5侧翼序列。并以此侧翼序列为标签继续用锚定pcr法向两边延伸，克隆出一个5045bp的dna片段。 |
| 6. | The first 24 amino acid residues of the n - terminal sequence of ba - dfe were aqsvpygvsqikapalhsqgftgs , which is different from those fibrinolytic enzymes , such as nk ( nattokinase ) , ck ( a fibrinolytic enzyme from chungkook - jang ) and kk ( katsuwokinase )
Ba - dfe能直接降解纤维蛋白，不能激活纤溶酶原形成纤溶酶。蛋白质测序结果显示，该酶n -端24个氨基酸序列为aqsvpygvsqikapalhsqgftgs 。 |
| 7. | Sequence analysis showed highly similarity to other plants such as narcissus pseudonarcissus . , and they all possess a short highly conserved n - terminal sequence encoding a dinucleotide cofactor binding site . thus it is a new member of phytoene desaturase gene family
由此可认为该番红花八氢番茄红素脱氢酶基因在结构上与其它植物八氢番茄红素脱氢酶基因是同源的，是八氢番茄红素脱氢酶基因家族中的一个新成员。 |
| 8. | Not only sequence information from the c - terminus of proteins and peptides is of especial interest in the investigation of n - terminally blocked proteins and peptides , but also c - terminal sequence analysis can facilitate the production of more specific probes for gene cloning
C端与n端一样，在蛋白质分子结构分析中具有重要的地位，对其顺序测定具有重要的意义，不仅可以对n端封闭的蛋白质进行序列测定，而且对基因克隆有指导意义。 |
| 9. | The homologous analysis showed that the n - terminal sequence of dhap is identical to that of serine protease from another b . pumilus strain tyo - 67 and has high homology with those from other bacillus species . based on the homologous analysis , three pairs of primers were designed and synthesized
该序列与来自其它芽抱杆菌属菌株的丝氨酸蛋白酶的n一末端氨基酸序列有较高的同源性，并和另一株短小芽抱杆菌的丝氨酸蛋白酶n一末端氨基酸序列完全一致。 |
| 10. | But in recent developments , especially in derivatizing reagents , have demonstrated that this method appears very promising in the field of protein c - terminal sequence analysis in future . furthermore , with the significant improvements of mass spectrometry technologies , which facilitate analysis of large biological molecules
同时近十年来随着质谱技术的发展，使得质谱在分析大分子化学物质成为可能后，在蛋白质顺序分析领域里取得了很多重要进展。 |
